Our Services

Protein Identification

MS3 Solutions provides a wide range of sample preparation solutions to suit your research requirements. From raw product to purified protein we can help identify proteins of interest. If your project requires a more detailed analysis, please contact us to discuss. We will provide you with a customised project plan to meet your research needs.

Sample Preparation Service

Protocols for preparing protein samples may differ according to the source of the samples, physical properties, subcellular location, protein abundance, and matrix that they are suspended in.

In our general workflow, protein complexes are separated by 1D or 2D gel electrophoresis and the target protein is then extracted and digested into peptide fragments, followed by HPLC separation and LC-MS/MS analysis.

MS3 Mass Spec Workflow.png

Mass Spectrometry

Protein identification using mass spectrometry takes many forms and is often dependent upon purity, complexity of the protein sample, and the type of coverage required. MS3 performs protein identification services for a broad range of sample types including:

  • Accurate mass determination of Intact proteins

  • Native proteins, recombinant proteins, and antibodies

  • 1D- and 2D-PAGE purified protein samples (Coomassie G250 stained proteins)

  • Affinity-purified (AP) and Immuno-purified (IP) samples

  • Fractionated lysates from animal tissue and fluids, plants, microorganisms, biofluids, and cells cultures


Analysis of Purified Protein Samples:

Purified proteins can be analysed by LC-MS (intact) or by LC-MS/MS (bottom-up). MS3 uses Orbitrap
UHPLC LC-MS/MS technology to provide high-sensitivity detection of purified protein samples. For full sequence coverage, MS3 offers a multi-enzyme approach for peptide mapping studies.


Analysis of Complex Protein Samples:

For more complex samples MS3 can employ extended LC gradients or sample fractionation techniques such as isoelectric focusing, 1D/2D PAGE, selective precipitation or affinity/immuno-chromatography prior to mass spectrometry. Talk to us if you require a more detailed analysis of a complex sample.

MS3 uses PEAKS Studio X bioinformatic software for all analyses providing:

  • de novo sequencing

  • Database search

  • Spectral Library Search

  • Post-translational modification (PTM) search with 500+ modifications

  • Sequence variant and mutation search

  • Protein Quantification (Label-free and TMT)

 

We provide fast and accurate results at affordable prices. For straightforward analysis, please download the sample submission form.